Leprosy Diagnostic Landscape

Slit-Skin Smear Microscopy (Ziehl–Neelsen)

Direct enumeration of acid-fast bacilli in Ziehl–Neelsen-stained dermal smears, yielding the Ridley logarithmic bacterial index used for operational classification and for monitoring bacillary clearance during multidrug therapy.

Technology
Microscopy & Histopathology · Direct microscopy
What it measures
Acid-fast bacilli (bacterial index)
Developer
In-house and national programmes
Specimen
Slit-skin smear
Development status
FDA/CE/WHO endorsed
Regulatory status
National approval
Intended use
Operational classification and monitoring of bacillary clearance during multidrug therapy
Main limitation
Insensitive in paucibacillary disease and cannot tell M. leprae from other mycobacteria.
Linked publications
837
TPP-1, confirming leprosy
40.5 of 100, partial alignment
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