Multi-Target Multiplex qPCR (RLEP, 16S rRNA, sodA and hsp18)
Simultaneous amplification of RLEP with 16S ribosomal RNA, sodA or hsp18 targets. Multiple targets recover part of the sensitivity deficit in paucibacillary specimens, and RNA targets permit inference of bacillary viability.
- Technology
- Molecular (NAAT) · Real-time PCR
- What it measures
- Multiple M. leprae genomic and transcriptomic targets
- Developer
- Multiple laboratories
- Specimen
- Slit-skin smear
- Development status
- LDT with clinical validation
- Regulatory status
- None / RUO
- Intended use
- Confirmation with improved paucibacillary yield and inference of bacillary viability
- Main limitation
- A more complex workflow, validated mostly at single sites.
- Linked publications
- 7
- Collaborating institutions
- Fiocruz and the Instituto de Biologia Molecular do Paraná (Brazil), Stanley Browne Laboratory, The Leprosy Mission Community Hospital (Delhi, India), Schieffelin Institute of Health Research and Leprosy Centre (Karigiri, India), Postgraduate Institute of Medical Education and Research (Chandigarh, India), ICMR-National JALMA Institute (Agra, India), Institute of Dermatology, Chinese Academy of Medical Sciences (Nanjing, China), Royal Tropical Institute, KIT (Amsterdam, the Netherlands)
- TPP-1, confirming leprosy
- 33.0 of 100, low alignment
- TPP-2, infection in contacts
- 27.5 of 100, low alignment
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