CRISPR-Cas12/Cas13 Detection
Guide RNA-directed activation of a Cas nuclease, with collateral cleavage of a reporter substrate, coupled to isothermal pre-amplification. The recognition step confers single-nucleotide discrimination, permitting combined detection and resistance genotyping.
- Technology
- Molecular (NAAT) · CRISPR diagnostics
- What it measures
- M. leprae DNA/RNA with collateral cleavage read-out
- Developer
- Laboratory-developed (no single developer)
- Specimen
- Slit-skin smear
- Development status
- Assay in development
- Regulatory status
- None / RUO
- Intended use
- Combined detection and resistance genotyping with single-nucleotide discrimination
- Main limitation
- No published leprosy study, so performance is estimated from work on related mycobacteria.
- Linked publications
- TPP-1, confirming leprosy
- 39.0 of 100, partial alignment
- TPP-2, infection in contacts
- 35.0 of 100, partial alignment
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